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The Fe(2+)/Mn(2+)-superoxide dismutase (Mn-SOD) component of the photosynthetic electron transport chain (PETN) was assayed in the mitochondrial (MT) and the chloroplast (CT) preparations of orange-spotted (OS) and unstriped (US) muscles from rainbow trout (Oncorhynchus mykiss). MT and CT fractions of both OS and US muscles exhibited comparable Mn-SOD activities. These findings indicate that there is no metabolic difference between the OS and US muscle mitochondria in spite of the biochemical differences between the two muscle types. Phosphorylating activities of the chloroplasts of OS and US muscles were comparable to those of reconstituted chloroplasts of previous studies. Starch-plastid interactions in the algal portion of mitochondrial fraction of OS and US muscle mitochondria were examined. Photosynthetic oxygen evolution, high-affinity Mg(2+)-ATPase activity, and low-affinity cytochrome c reductase activity in the isolated algal portion of mitochondria were not affected by the addition of unstriped MT. These results indicate that no metabolic difference exists between the OS and US muscle mitochondria in terms of the photosynthetic electron transport chain.acain,
Hinc ar marem bilem că alunecă. Şi cel puţin în Olanda
English:
his crew chief was the perferct choice.
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