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Password.txt 1.4 KB.rar
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Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 Kb
Download Winrar password remover 1 1 rc rar from 4shared
Password.txt 1.4 K
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Tags:
android,. RAR Password Unlocker, password generator, password txt, password recovery,. RAR Password Cracker, password txt 1.4kb,.
Time:
2010-12-05
Posted by:
kvnk
04/26
BUY ANDROID ONLY APPS, VIDEO, MUSIC, ETC. AND NOT UPDATES, DATA, CALL LOGS.Reconstitution of a novel maternal MT nucleoprotein as a molecular chaperone for ovine SRSF2.
Spatiotemporal expression patterns of the maternal mammalian translational machinery are essential for the specification of oocyte developmental competence, which is associated with complex temporal and spatial regulation of maternal mRNAs. Here, we have identified a novel RNA-binding protein with high sequence similarity to the C-terminal RNA recognition motif (RRM) of SRSF2 that, during early oocyte development, is expressed on the surface of the female pronucleus. This protein is localized along the inner membrane of the oocyte cytoplasm, where it co-localizes with SRSF2 and eIF4E, and is present on a substantial fraction of mouse and sheep maternal mRNAs. The C-terminal RRM of SRSF2 is necessary and sufficient for interaction with RNA through a novel penta-arginine motif. Using an affinity-purified antibody, we demonstrate that this novel protein is overexpressed following IVF in oocytes and embryos of various mammalian species and at different developmental stages. Analyses of immunoprecipitated particles demonstrated that the endogenous SRSF2, and an engineered RRM-deleted version, binds the immunogen to the same high affinity, and that the purified protein specifically binds RNA. We also show that the affinity of the native protein for RNA is high enough to bind specifically to the oocyte Ran guanine nucleotide exchange factor (RanGEF). Reconstitution experiments demonstrate that SRSF2 can enhance RNA-binding activity of the RanGEF, even in the absence of other factors present in the oocyte cytoplasm. The developmentally regulated expression patterns of SRSF2 and the RNA-binding activity of this protein may help to regulate a large pool of maternal mRNAs in oocytes.Lee Kuan Yew Education Foundation
The Lee Kuan Yew Education Foundation () is a
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