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Pseudomonas aeruginosa is one of the most important opportunistic pathogens, and a leading cause of morbidity and mortality in cystic fibrosis (CF) patients. To gain insights into the interaction of this important pathogen with host innate immune defence cells, we quantitatively analysed the in vitro interaction of clinical isolates of P. aeruginosa and Pseudomonas putida (that is present in the CF lung) with human primary neutrophils (PMN). Overall, we observed a significantly higher binding affinity of P. aeruginosa clinical isolates compared with P. putida. Using a real-time imaging analysis of neutrophil-P. aeruginosa interaction, we show that the clinical isolates preferentially induce degranulation of the neutrophils. Moreover, neutrophil-mediated killing of P. aeruginosa was elevated with both the clinical isolates. Taken together, we demonstrate that P. aeruginosa clinical isolates show a greater capacity to interact with PMN, even though the initial contact and subsequent killing does not seem to differ between clinical isolates and P. putida. We propose that these interaction differences may be of biological significance in the microbial pathogenesis and/or host defence of P. aeruginosa.Effects of a brief intervention on cervical touch referral rates for pregnancy loss.
To determine whether (1) a brief, provider-initiated, office-based cervical touch referral intervention increases the rates of cervical examination during pregnancy losses, (2) the rates of cervical examination after pregnancy losses differ between office and private diagnostic ultrasound (US) facilities, and (3) any differences in cervical examination rates after pregnancy losses according to time of day of loss and length of time since loss are explained by initial referral rates to ultrasound facilities. After IRB
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17th Jan In this video I demonstrate the Dungeon Dimensions Master. First you build a hex map on a piece of paper, then you “unveil the dimensions”. The dimensions (barriers, traps, rooms etc) become physical obstacles that push the hero in a certain direction when they are attacked. The hero has no problem moving along the invisible lines of “shadow”, since he is not aware of it. I recommend this game for all adventures rooms. The Hero is no longer a boring limited size being. The game is meant to be played with very little instruction. It’s up to the GM to master the game and provide some guidance to the players. The Hero begins at the origin of our hex map and the villain resides at the end. The Hero has 3 attributes : size, strength and intelligence, they go up as the Hero goes. Each time the Hero attacks a monster or opens a door he gets more attributes. But when the hero fights the final boss he is so powerful he is supposed to add more attributes to the time.
One of my friends who is an RPG player and decent Gaffer, got a copy of John Wick Presents and had me (me being the wife) give it a go on the 12th May as we played a game. After a week, I have played for 4 hours, so far. I found it to be a brilliant game, and looking to expand my horizons, have booked it up for the Bladerunner conventions mid June.
I don’t think there’s anything like this for 4 players. The game is easiest to play, and most enjoyable, when there are at least 2 players who know enough of D&D to be happy playing one or two adventures a day. How to even get playing in the first place? 4 players isn’t even a full party. 1 player must be designated as the DM. It’s the DM who must know how to run a game properly and communicate with the players well enough to make the entire experience enjoyable for everyone. If I was running a one or two-person game, I’d definitely do it the D&D way as well, but as a DM I’d find it strange.
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