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Lineage-specific histone H3 acetylation on the human immunoglobulin heavy chain enhancer region during B cell development.
Expression of the immunoglobulin heavy chain (IgH) gene is strictly regulated at the level of transcription initiation. To study in detail the mechanisms that control the tissue- and differentiation-specific expression of the IgH gene, we have examined the in vivo acetylation pattern of the DNA-binding proteins by specific antibodies in murine and human B cells. In embryonic day (E) 13.5 murine fetal liver cells, we have localized the IgH histone H3 acetylated lysines 14 and 27 in the human IgH enhancer region near the transcription start site. In this developmental stage, mononuclear cells lacking surface immunoglobulin expression showed a lack of histone H3 acetylated lysines 14 and 27. In contrast, cell-surface IgM+ pro-B cells, in which the IgH gene was transcriptionally active, showed a significantly high level of histone H3 acetylated lysines 14 and 27. Thus, we have identified that the IgH histone H3 acetylation is lineage-specifically regulated in vivo.Glasgow brothers jailed for life for murdering Glasgow lawyer
25 February 2016
A verdict of guilty was reached today in the trial of two brothers accused of the murder of Glasgow-based lawyer Nicky Patrick.
Leonard Patrick, 41, and Leroy Patrick, 37, both of Glasgow, were convicted by the Glasgow Sheriff Court jury of the murder of Patrick, who was shot and killed on Millacre Road, Clydebank, on January 31 last year.
After the conclusion of a three-week trial, a forensic expert said the shell casings found at the scene were linked to the same gun that fired the bullets that hit Patrick.
Jurors were also told that the brothers used Patrick’s car to block the road, before entering the vehicle and fleeing the scene.
Police
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