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In summary, our study suggests that vitamin D deficiency can cause impaired thymic T-cell development by blocking the maturation of thymic epithelial cells, which leads to the transformation of regulatory T cells and the acceleration of tumour development and metastasis. Future studies should focus on the upstream regulatory mechanism in the thymus that controls the maturation of thymic epithelial cells. There is no doubt that we should find new strategies to overcome vitamin D deficiency to prevent cancer. Our research will benefit from collaboration with others, and research is progressing rapidly in this field.
MATERIALS AND METHODS {#s4}
=====================
Cells, plasmids, and antibodies {#s4_1}
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The human colorectal cancer cell line (HCT116) and the human colorectal cancer cell line (HCT8) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The mouse colorectal cancer cell line (CT26) was obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). RPMI 1640 medium, Fetal bovine serum (FBS), and Dulbecco’s Modified Eagle Medium (DMEM) were purchased from Gibco. The following antibodies were used: anti-human FOXP3 (Santa Cruz); anti-mouse CD4, CD45, CD25, CD11c, CD80, CD86, FOXP3, TCRγδ, CD3, CD8 (eBioscience); anti-human CD31 (BD); phycoerythrin (PE)- and allophycocyanin (APC)- conjugated CD3 (Beckman Coulter); and anti-phospho p38, phospho-p42/p44, phospho-JNK, and
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