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This is a classic, modern style house built on a picturesque site in the village of Sagrario in Umbria, Italy.
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This is a classic modern style house built on a picturesque site in the village of Sagrario in Umbria, Italy.
This is a classic modern style house built on a picturesque site in the village of Sagrario in Umbria, Italy.
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The aim of the study was to evaluate the immune response of broiler chickens to oral infections with Salmonella Gallinarum or Salmonella Enteritidis and peroral administration of probiotic bacteria. Tonsils were taken from chicks at days 3, 5 and 10 after the first infection and the number of lymphocytes, differential count and cytotoxicity of T lymphocytes were determined. Lymphocytes from the spleen were transferred in vitro to measure the in vitro response of lymphocytes to specific antigen of T-dependent and T-independent type. The in vitro response of lymphocytes to specific antigen was measured with the MTT assay. The results showed that the 1st and 2nd infection with Salmonella caused changes in the immunological characteristics of chickens: decreased number of T lymphocytes and cytotoxic activity of T lymphocytes, increased number of T8 lymphocytes. In the 1st infection there were no differences in the number of lymphocytes in chickens which had received Lactobacillus acidophilus as a probiotic and those which did not, and a decrease in the number of lymphocytes in chickens of the second group. In the 2nd infection, there were no differences in lymphocyte number in chickens receiving probiotics and those receiving the placebo as well. The 2nd infection with Salmonella did not significantly change the number of T lymphocytes in chickens treated with probiotics. Also in this group a decrease in the number of T lymphocytes was observed. The efficiency of the probiotic administration depended on the kind of salmonella and period of observation. The in vitro response to Salmonella was detected earlier in the 2nd infection than in the 1st. The 2nd infection with Salmonella led to the decrease in the number of T lymphocytes in chickens receiving probiotics, which indicated
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