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We recently reported that the induction of tumor-antigen-specific CD8+ T cells in tumor-draining lymph nodes (TDLNs) is a critical part of the mechanism of tumor-specific antitumor immunity. The induction of TDLN-antigen-specific CD8+ T cells was only observed with intralymphatically (IL) but not intravenously (IV) injected vaccines. The aim of this study was to determine the most effective tumor antigen-primed and -pulsed antigen-presenting cell (APC)-based vaccine regimen to induce TDLN-antigen-specific CD8+ T cells. To this end, we used a TC-1 tumor model to establish the optimal TDLN-antigen-specific effector function of two novel peptide-pulsed antigen-presenting cell-based vaccine regimens: IV/TDLN and IL/TDLN. We also established the optimal TDLN-antigen-specific effector function of two novel peptide-pulsed antigen-presenting cell-based vaccine regimens: IV/TDLN and IL/TDLN. We demonstrated that IL/TDLN was significantly superior to IV/TDLN in inducing TDLN-antigen-specific effector function and that there was a dose correlation between the coadministered number of antigen-pulsed antigen-presenting cells and the induced TDLN-antigen-specific CD8+
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