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In the sand fly Phlebotomus papatasi (Scopoli, 1786) (Diptera: Psychodidae) there is a QTL for oviposition-related venom secretion on the right arm of chromosome LGV. Previous genetic studies of this chromosome showed that the gene coding for the enzyme carboxypeptidase 3 is located within this QTL. Here we report the identification of the corresponding genomic sequence by transcriptional analysis of a cDNA prepared from the P. papatasi salivary glands. The cDNA and the predicted protein were named pep3 and the enzymes catalyzing carboxypeptidase and proteinase activities, respectively. The deduced amino acid sequences of pep3 and other relevant gene products from other insects are more similar to mammalian orthologs than to each other. The transcriptome analysis revealed a total of eight genes (seven in clusters) within the P. papatasi QTL. The topography of the clusters and the presence of a partial gene coding for a putative serine protease all point towards a tandem duplicated region. The data presented here provide the first candidate genes for a QTL regulating oviposition-related venom secretion in any insect and provide further insight into the evolution of toxin-related genes.Preventing human immunodeficiency virus (HIV) infection and other STDs is an important public health priority in developing countries. The risk of acquiring HIV infection is estimated to be 1-3 times higher in women than men, and the greatest risks are among persons who have multiple sexual partners, particularly women. In Thailand, the prevalence of HIV infection and sexually transmitted disease (STD) is highest in women, particularly in the 15-24 year age group where the infection rates are over 30%. Recent cross-sectional data from the Thai RNTCP show that women have a higher prevalence of HIV infection than men (21% vs 12%), and the rate of new HIV infection is nearly 40% higher in women (
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