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A diagrammatic illustration of the effect of diet quality (kJ/kg diet) and mastitis (no versus yes) on milk production, fat and protein content and milk yield is shown in Figure [2](#Fig2){ref-type=”fig”}. All interactions were significant. The highest milk yield and highest milk fat content occurred when mastitis was absent and diet quality was relatively low. In this case, milk yield was 6.4 kg/d higher, fat content was 1.2 g/100 g higher and protein content was 0.12 g/100 g higher than in the control cows. For the cows producing milk for 2.8 kg/d, the highest milk yield was achieved with mastitis (without) and relatively high diet quality. In the low milk producing cows (producing milk of 1.0 kg/d), mastitis (with) and low diet quality gave the highest milk fat content. The milk fat yield of the cows which produced milk for 2.8 kg/d was about the same with both mastitis and low diet quality. For protein content, highest values were achieved with mastitis (without) and relatively low diet quality. This was highest in low milk producing cows (producing milk of 1.0 kg/d).Figure 2**Milk fat, protein and milk yields (kg/d) of heifers \<9 months old according to mastitis (no = solid, yes = broken) and intake (low, medium, high) of a low energy diet (kJ/kg diet).** Error bars represent standard errors. Discussion {#Sec11} ========== In this study, the effect of mastitis on the production and composition of milk was examined during three different diets. As expected, mastitis generally reduced the production and composition of milk and protein content tended to be lower when mastitis was present. Although the data for fat content and yield were not normally distributed, the results of the linear regression analysis agreed with those of the generalised linear models. The random treatment effects were thus in agreement with those of the
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