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Chromatin condensation and the transition between interphase and metaphase in human epitheloid carcinoma cells induced to undergo programmed cell death by deprivation of essential amino acids. Ploidy, interphase, and metaphase structures were determined in EJ3056 cells induced to undergo programmed cell death by a 20-hr deprivation of essential amino acids. In contrast to previous data for terminally differentiated Leydig cells, this induced cell death was accompanied by metaphase chromatin condensation. Apoptosis was not inhibited by cycloheximide, cycloheximide plus actinomycin D, or the Ca2+ ionophore A23187. It was not accompanied by disruption of microtubules. The results indicate that the apoptosis in EJ3056 cells has a cytogenetic and biochemical basis distinct from that of apoptosis in terminally differentiated Leydig cells.// Copyright (c) 2012-2018 Ugorji Nwoke. All rights
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