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The desire to better understand cellular behaviors in the context of current biomaterial design has led to the development of sophisticated techniques to measure cell behaviors on various materials. These techniques are typically used in a time-consuming, less informative manner. To make the data obtained more readily interpretable, this study attempts to develop a technique that uses a simple, intuitive means to quantify the differences in cell adhesion and growth between smooth and nanorough surfaces. The study investigates the consequences of nanorough surface on osteoblast adhesion and cell morphology. Results show that cells on the nanorough sample are more spherical in shape, more spread, and exhibit a greater degree of contact. These data demonstrate that the technique used in this study can be used to quantitatively measure a morphological feature associated with cell function.[Evaluation of a prognostic score for patients with primary breast cancer receiving radiotherapy after breast-conserving surgery].
To evaluate the prognostic score (PS) for Chinese patients with primary breast cancer undergoing radiotherapy after breast-conserving surgery (BCS), by using the Cox regression model. The subjects were enrolled from January to July 2005. The prognostic value of clinicopathologic factors for patients with breast cancer was analyzed and the PS was developed, using stepwise multivariate analyses. The PS was calculated based on the hazard ratios of the Cox regression model. Survival was analyzed with Kaplan-Meier method. The PS included age, lymph node status, tumor size, estrogen receptor (ER) expression, Ki67 expression and treatment site. A total of 272 patients were analyzed. The 5-year disease-free survival (DFS) and overall survival rates were 70.7% and 84.8%, respectively. Both the DFS and overall survival were found to be better in the positive ER group (P = 0.028 and 0.028, respectively). The 5-year disease-free survival and overall survival rates of patients with a PS ≤ 6 were 75.3% and 84.7%, respectively
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