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Transcriptional activation by GATA transcription factors is dependent on interactions of the GATA DNA-binding motif with the minor groove of DNA. The BOM promoter contains two putative GATA motifs, but the mode of gene activation is unknown. We report that two GATA4/BOM TATA box motifs function as a bipartite platform to activate GATA4 gene transcription in vitro and in vivo. The GATA4/BOM TATA box motifs direct transcriptional activity in vitro, and mutations in both sites that abolish a third consensus GATA motif also abolish transcriptional activation of the GATA4 gene in vivo. The functional GATA motifs are required for transcriptional activity of the GATA4 gene in vivo, as determined by the effects of site-specific mutations in mice. Furthermore, promoter substitution experiments demonstrate that the two GATA motifs function independently of each other. We propose that the GATA4/BOM TATA box motifs provide the first example of a tetrameric DNA-binding motif that functions as a bipartite platform for the regulation of transcription.Podcast
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Windows Server 2012 R2, two WebApplications on one DC
I need to have two web applications running on one Web Edition Server instance. I already have the machines created and the Server is running Windows Server 2012 R2 (Web Edition – Express Server). I can’t use any other edition because this server is going to be used in a production environment.
I guess I need to edit the System.configuration so the two IIS can see each other, but I can’t find the connection to do it.
I need to have for instance 2 web applications in one domain (selfhosted) and I can’t use sharepoint as the clients are Non-Microsoft Application.
EDIT : I have this as my connection string : `
A:
You can’t. A Web Edition server doesn’t allow you to create more than one web site per instance.
There may be Windows 2012 Standard or Ultimate editions that do allow you to have multiple web sites/virtual servers.
If you only need
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