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. Title: Ò¬hosÒ½ who liÒ´Ò½ to sharÒ´Ò½ thÒ´Ò½r photos and moviÒ´Ò½s taÒ´Ò½n, for Ò´Ò´Ò´ªxamplÒ´Ò´Ò´Ò´â€, duringâ€Â .Identification of a fusion transcript, t(9;22), in a Burkitt lymphoma cell line.
A variant translocation t(9;22)(q13;q12) has been found in a low grade B-cell lymphoma cell line (BL 3.18). The translocation has not previously been reported. This variant translocation was first identified by Southern blot analysis of restriction digests of total genomic DNA. In order to demonstrate that this rearrangement is consistent with the variant t(9;22), Southern analysis of total and poly(A)+ RNA separated by agarose gel electrophoresis was performed. The results revealed a specific fusion transcript of the type expected for the variant t(9;22). This is the first report of a variant t(9;22) and a specific fusion transcript of this type.The mechanism of the inactivation of acylpeptide hydrolases by bivalent zinc.
Purified acylpeptide hydrolases from pig liver and hog kidney were inactivated by acetylation of the Zn2+ atoms in the enzyme molecule at different stages of purification. Zn2+-dependent acylpeptide hydrolase inactivated by treatment with 100 microM N-ethylmaleimide became reactivated after removal of the modification by dithiothreitol. Kinetic analyses of the reactivation and of the partial reacivation suggested that the inactivated Zn2+ is present in the enzyme in the form of a complex of the enzyme with a polypeptide containing a single modification of a Thr residue. This inactivated enzyme was reactivated by Ca2+, N-ethylmaleimide, and a sulfhydryl reagent (p-chloromercuribenzenesulfonate). The reactivated enzyme showed a pH optimum of 6.0-6.5 and an increased Km for substrate peptide from 21 to 72 microM. It was also effective on natural substrates.
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To evaluate the feasibility of reducing the practice of intensive care unit (ICU) for patients under 20 years old. This study was conducted in the patients under 20 years old who admitted to ICU of Beijing Children’s Hospital (BCH) between January 1, 2016 and June 30, 2016. Age, weight, height, APGAR score, and survival rate were recorded. The patients were divided into two groups: a control group (group I, 36 patients) and a reduction group (group II, 35 patients). The data was compared between two groups, and survival rates were compared. There was no difference in age, weight, height, APGAR score, and survival rate between two groups. All of the patients who accepted restrictive
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