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During intense muscle activity, intracellular Ca2+ release from the sarcoplasmic reticulum (SR) by Ca2+-release channels can generate a local Ca2+ wave of sufficient amplitude to activate the contractile machinery. This function was demonstrated for the first time in skinned muscle fibers in a study by Thompson et al. (1981). Subsequent work, however, has shown that also cultured muscle cells are able to generate local Ca2+ waves under appropriate experimental conditions (e.g., using local injection of Ca2+-releasing agents). The aim of this review is to describe some of the features of this new function and to see how it may contribute to the normal functioning of muscle. The sarcoplasmic reticulum, composed of luminal reticulum vesicles and tubular membranes connected to the cell membrane, plays a fundamental role in muscle contraction by providing Ca2+ stores from which a local elevation of free [Ca2+] may be generated by Ca2+ release channels. Under normal conditions, the sarcoplasmic reticulum may generate waves of Ca2+ elevations or fluctuations due to Ca2+ release in response to excitation-contraction coupling or by release of Ca2+ caused by inositol-1,4,5-trisphosphate action. The phenomenon of Ca2+ release waves may be considered as a new function of the SR in muscle which is analogous to the calcium spikes generated by cells in the central nervous system. It is therefore proposed that the SR plays a role in transmitting local signals between cells in muscle.Q:
Azure Service Fabric Cluster won’t run after update
I have updated my cluster. Now I can’t deploy the service anymore since it’s still connected to the old instance (default service).
I tried to reset the datastore to empty in the config settings but it also won’t seem to work. It’s not recognized anymore.
What should I do in this scenario?
A:
While it is working correctly, it is keeping that old instance alive for a few more minutes so that it can finalize all of the “recent” actions.
In the mean time, in Azure Portal, the cluster overview screen will
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